Correlation between microsomal lipid peroxidation levels and drug metabolizing enzymes in rats on various ages |
Jong-Hoo Cho1, DaeWoo Hwang2, Sang-Youel Park1 |
1Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University 2SungBo Science Tech. |
연령증가에 따른 마이크로솜 막지질 과산화수준의 변화와 해독효소계의 관계 |
조종후1, 황대우2, 박상열1 |
1전북대학교 수의과대학, 생체안정성연구소 2(주)성보사이언스텍 |
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Abstract |
The studies were carried out on the correlation between microsomal lipid peroxidation level and drug metabolizing enzyme activities in rat liver microsomal suspensions on various ages (2-week-old, 2, 4, 8, and 12-month-old). The lipid peroxidation levels of liver homogenates tended to be elevated in a 4-month-old rat livers, but it was a little decreased in 8 and 12-month-old rat livers. The lipid peroxidation levels of microsomal suspension was not shown any significant differences by ages. Lipid peroxidation levels and microsomal cytochrome P450 and NADPH-cytochrome c reductase activity showed a direct correlation (r=0.72 and r=0.64), respectively. The activities of cytochrome P450-dependent aminopyrine-N-demethylase and benzpyrene hydroxylase in rat liver microsomes were increased by ages up to 8-month-old rats and maintained in 12-month-old rats. The correlation between lipid peroxidation levels and these cytochrome-dependent enzyme activities showed a high direct correlation (r=0.97 and r=0.81), respectively. |
Key Words:
microsome, cytochrome P450, drug metabolizing enzymes |
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