Korean Journal of Veterinary Research 2004;44(1):41-47.
Analysis of gamma-ray-induced DNA damage in human, mouse and rat peripheral blood lymphocytes using single-cell gel electrophoresis
Heon Oh1, Uhee Jung1, Hae-Ran Park1, Sung-Ho Kim2, Sung-Kee Jo1
1Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute
2College of Veterinary Medicine, Chonnam National University
단세포 전기영동법을 이용한 인체, 마우스 및 랫드 림프구의 방사선에 의해 유발된 DNA 손상 측정
오헌1, 정우희1, 박혜란1, 김성호2, 조성기1
1한국원자력연구소 방사선식품.생명공학팀
2전남대학교 수의과대학
The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.
Key Words: single-cell gel electrophoresis (SCGE), comet assay, lymphocyte, human, mouse, rat, radiation
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