Korean Journal of Veterinary Research 2004;44(2):159-162.
Chromosomal band pattern of black-striped field mouse (Apodemus agrarius)
Seung-hyun Oh1, Yeo-sung Yoon2, Hee-Kyung Jin3, Je-kyung Seong1
1Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University
2Laboratory of Histology, College of Veterinary Medicine, Seoul National University
3Laboratory of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University
한국 야생 등줄쥐의 고해상도 염색체분염상
오승현1, 윤여성2, 진희경3, 성제경1
1서울대학교 수의과대학 발생.유전학교실
2서울대학교 수의과대학 조직학교실
3경북대학교 수의과대학 실험동물학실
Abstract
We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.
Key Words: black-striped field mouse, karyotype, GTG-bands


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