| Quantitative assay of glycosphingolipid and determination of binding affinity for Shigatoxin 2e in pig |
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Bun-youn Dong1, Sang-Hyun Kim2, Hyun-Ho Cho3, Yeong-Il Kim3, Woo-won Lee4, Kon-Sup Kim5, Ho-Jo Kang5, Yong-Hwan Kim5 |
1Korea Green Cross Co.
2Department of Pathobiology, Ontario Veterinary College, University of Guelph
3National Veterinary Research and Quarantine Service
4Pusan Institute of Health and Environment
5College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University |
| 돼지의 일령에 따른 glycosphingolipid의 정량 및 Shigatoxin 2e에 대한 친화성 확인 |
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동분연1, 김상현2, 조현호3, 김영일3, 이우원4, 김곤섭5, 강호조5, 김용환5 |
1녹십자백신연구소
2캐나다 구엘프대학교 수의과대학
3수의과학검역원 서울지소
4부산시 보건환경 연구원
5경상대학교 수의과대학 생명과학연구소 |
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| Abstract |
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This study was designed to quantitative assay of cellular receptor globotriosyl ceramide($Gb_3$) and globotetraosyl ceramide($Gb_4$) and to determine binding affinity of the receptors for Shigatoxin 2e in difference with day olds of pig. The amount of $Gb_3$ and $Gb_4$ extracted from each swine tissue were detected in order of spleen, ileocecum, kidney, and jejunum. The amount of $Gb_3$ and $Gb_4$ extracted from each swine ileocecum and jejunum in different day old swine were detected in order of 24, 6, 98, 12 and 35, but the difference of amount was not significant. The binding affinity of $Gb_4$ extracted from ileocecum and jejunum with different day old swine for Stx2e were shown that the extracts of 24, 12, and 35 days old swine were higher significantly than that of 6 and 98 days old swine. |
| Key Words:
Globotriosyl ceramide, globotetraosyl ceramide, Shigatoxin 2e, receptor binding affinity |
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