| Survey of antibody on Orientia Tsutsugamushi among wild rodents in Gyeongnam area and detection by nested polymerase chain reaction |
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Dae-Sik Hah1, Young-Hoon Kim1, Jung-Ung Park1, Jae-Kap Park1, Chung-Hui Kim2, Jae-Doo Ryu3, Myung-Ho Jong4, Jung-Ho Heo4, Jong-Lip Shu4, Myung-Heui Cho4, Kuk-Cheon Lee4, Gon-Sup Kim5, Eui-Kyung Kim5, Jong-Shu Kim5 |
1Gyeongnam Provincial Government Institute of Health and Environment
2Department of Animal Science and Biotechnology, Jinju National University
3National Veterinary Research and Quarantine service Busan Regional Office
4Gyeongnam Livestock Promotion Institute South-branch
5Agriculture & Life Science, College of Veterinary Medicine, Gyeongsang National University |
| 경남지역 야생들쥐에서 Orientia tsutsugamushi에 대한 항체 조사 및 PCR에 의한 검색 |
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하대식1, 김영훈1, 박정웅1, 박재갑1, 김충희2, 류재두3, 정명호4, 허정호4, 서종립4, 조명희4, 이국천4, 김곤섭5, 김의경5, 김종수5 |
1경남보건환경연구원
2진주산업대학교 동물생명과학과
3국립수의과학검역원 부산지원
4경남축산진흥연구소 남부지소
5경상대학교 수의과대학 농업생명과학원 |
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| Abstract |
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As a part of epidemiologic investigation of tsutsugamushi disease, the wild rodents which were captured in Gyeongnam area were diagnosed with indirect immunofluorescent antibody assay (IFA) and Polymerase Chain Reaction (PCR) to find if they have an antibody against Orientia tsutsugamushi. The conclusion was drawn as followings. (1) The captured 58 wild rodents showed that the subspecies distribution of Apodemus agrarius was 86.2%, Microtus fortis was 8.6% and Crocidura lasiura was 5.2%. (2) The antibody positive rate of O. tsutsugamushi Gilliam, Karp, Karto and Boryong by IFA method was 32.0% in Apodemus agrarius among 50 wild rodents and 40.0% in Microtus fortis among 5 wild rodents, respectively. It was negative in the case of all the 3 Crocidura lasiura. (3) The antibody titers on Apodemus agrarius, Microtus fortis and Crocidura lasiura against Gilliam, Karp, Karto and Boryong were measured between 1:20 and 1:640. The antibody titer against each antigen was in the order Boryong>Gilliam>Karp. (4) O. tsutsugamushi was detected from the blood, spleen and kidney from the artificially infected mice by IFA and PCR. IFA showed the positive response between 3 and 18 days after inoculation. On the other hand, positive response was found from all the samples by PCR. (5) From PCR of the genomic DNA extracted from the blood, spleen and kidney samples of the captured wild rodents, Boryong-specific amplification product with size of 210 bp, which is particular in Boryong, was detected from spleen and kidney samples, but not detected in the blood. (6) Boryong-specific amplification product was detected from spleen and kidney samples which were obtained at 3, 6, 12, 18 and 24 days after the infection with Boryong. But, it wasn't detected from the uninfected samples. (7) From PCR of spleen and kidney samples of the captured wild rodents, it was found that positive rate of O. tsutsugamushi in Apodemus agrarius and Microtus fortis were 25.0% (4/16) and 20.0% (1/5), respectively. From the above results, it can be concluded that Apodemus agrarius resided in Gyeongnam area carried O. tsutsugamushi and PCR method might be a simple, precise, rapid and useful diagnostic tool than IFA for the diagnosis of O. tsutsugamushi. |
| Key Words:
Orientia, thutsugamushi, PCR, Gilliam, Boryong |
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