Korean Journal of Veterinary Research 2004;44(3):323-327.
Establishment of micronuclus assay as biological dosimetry in pig lymphocytes after gamma-irradiation
Se-ra Kim1, Hae-june Lee1, Jin-hee Lee1, Chang-mo Kang2, Tae-hwan Kim3, Sung-kee Jo4, Jong-choon Kim1, Sung-ho Kim1
1College of Veterinary Medicine, Chonnam National University
2Korea Institute of Radiological & Medical Science
3College of Veterinary Medicine, Kyungpook National University
4Korea Atomic Energy Research Institute
돼지 림프구의 미소핵 형성을 지표로 방사선 생물학적 선량측정법 확립
김세라1, 이해준1, 이진희1, 강창모2, 김태환3, 조성기4, 김종춘1, 김성호1
1전남대학교 수의과대학
2원자력의학원
3경북대학교 수의과대학
4한국원자력연구소
Abstract
The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and $4{mu}g/ml$ Cytochalasin B(Cyt-B) in pig at 72 hours after incubation. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was $y=0.0183D+0.0124D^2+0.0133$(y = number of MN/CB cells and D=irradiation dose in Gy). In conclusion, the results demonstrate that it appears feasible to use pig as target organisms in the micronucleus test to estimate the cytogenetic damage caused by ionizing radiations or, potentially, chemical compounds.
Key Words: micronuclei, pig, lymphocyte, radiation


About
Browse articles
For contributors
Policy
Editorial Office
#401-1, 85 Bldg., College of Veterinary Medicine, Seoul National University
1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea
Tel: +82-2-880-1229    Fax: +82-2-878-9762    E-mail: jvs@ksvs.or.kr                

Copyright © 2024 by The Korean Society of Veterinary Science.

Developed in M2PI

Close layer
prev next