Establishment of micronuclus assay as biological dosimetry in pig lymphocytes after gamma-irradiation |
Se-ra Kim1, Hae-june Lee1, Jin-hee Lee1, Chang-mo Kang2, Tae-hwan Kim3, Sung-kee Jo4, Jong-choon Kim1, Sung-ho Kim1 |
1College of Veterinary Medicine, Chonnam National University 2Korea Institute of Radiological & Medical Science 3College of Veterinary Medicine, Kyungpook National University 4Korea Atomic Energy Research Institute |
돼지 림프구의 미소핵 형성을 지표로 방사선 생물학적 선량측정법 확립 |
김세라1, 이해준1, 이진희1, 강창모2, 김태환3, 조성기4, 김종춘1, 김성호1 |
1전남대학교 수의과대학 2원자력의학원 3경북대학교 수의과대학 4한국원자력연구소 |
|
Abstract |
The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and $4{mu}g/ml$ Cytochalasin B(Cyt-B) in pig at 72 hours after incubation. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was $y=0.0183D+0.0124D^2+0.0133$(y = number of MN/CB cells and D=irradiation dose in Gy). In conclusion, the results demonstrate that it appears feasible to use pig as target organisms in the micronucleus test to estimate the cytogenetic damage caused by ionizing radiations or, potentially, chemical compounds. |
Key Words:
micronuclei, pig, lymphocyte, radiation |
|