Expression of osteopontin in developing mouse brain

Kim, Gyubeom; Hwang, Insun; Mun, Changjong; Shin, Taekyun; Son, Hwa-young; Jee, Youngheun

Korean Journal of Veterinary Research 2004;44(3):335-341.

Gyubeom Kim¹, Insun Hwang¹, Changjong Mun¹, Taekyun Shin¹, Hwa-young Son², Youngheun Jee¹

¹Department of Veterinary Medicine, College of Agriculture and Life Science, Cheju National University
²College of Veterinary Medicine, Chungnam National University

발달 중인 생쥐 뇌에서의 Osteopontin 발현

김규범¹, 황인선¹, 문창종¹, 신태균¹, 손화영², 지영흔¹

¹제주대학교 농업생명과학대학 수의학과
²충남대학교 수의과대학

Abstract

This study was undertaken to examine the developmental expression of osteopontin(OPN) in the mouse brain. In Western blotting analysis, the expression of OPN was noted initially at embryonic stage and increased gradually after birth and decreased at postnatal day 60(P60). In immunohistochemistry, OPN expression was found in the interstitial nucleus Cajal and the substantia nigra reticularis in anterior part of the brain and in the inferior olivary complex, the parabrachial nucleus, the facial nucleus, the gigantocellular reticular nucleus, the trigeminal nucleus and the anterior interposed nucleus in posterior part of the brain at P31 and P60. In addition, OPN expression in widespread neurons appeared during the period of neuronal differentiation, increased just after birth and decreased with maturation. These results suggest that OPN contributes to developmental processes, including the differentiation and maturation of specific neuronal populations.

Key Words: Osteopontin, developing brain, mouse