Korean Journal of Veterinary Research 2004;44(3):421-426.
Isolation and characterization of Brachyspira pilosicoli in Korean dogs
Tae-Jung Kim, Jae-Il Lee
College of Veterinary Medicine, Chonnam National University
국내의 개에서 Brachyspira pilosicoli의 분리 및 동정
김태중, 이재일
전남대학교 수의과대학
Abstract
This study was performed to report the outbreaks of canine intestinal spirochetosis and to characterize the canine isolates. Three canine isolates were weakly beta-hemolytic and had sharp end shape with 5 flagella. Isolates didn't produce indole but fermented fructose. In $API-ZYM^{(R)}$ study, isolates were alpha-glucosidase negative and alpha-galactosidase positive, which is the typical characteristics of B. pilosicoli. In multilocus enzyme electrophoresis(MEE) study, isolates were divided into 2 electrophoretic types. Isolates showed different properties with B. hyodysenteriae but closely related with pathogenic canine intestinal spirochete(B. pilosicoli). This is the first report of the isolation and characterization of canine intestinal spirochete in Korean dogs.
Key Words: Brachyspira pilosicoli, multilocus enzyme electrophoresis(MEE), Korean dog


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