The prevalence of swine influenza viral antigens and serum antibodiesin Piglets in Jeju

Jun, Yong-chul; Yang, Hyoung-seok; Yang, Na-yeoun; Kim, Dae-yong; Kim, Jae-hoon; Bae, Jong-hee

Korean Journal of Veterinary Research 2004;44(3):449-454.

Yong-chul Jun¹, Hyoung-seok Yang¹, Na-yeoun Yang¹, Dae-yong Kim², Jae-hoon Kim¹, Jong-hee Bae¹

¹Department of Veterinary Medicine, Cheju National University
²College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

제주지역 돼지에서 Influenza 바이러스 항원 및 혈중 항체 조사

전용철¹, 양형석¹, 양나연¹, 김대용², 김재훈¹, 배종희¹

¹제주대학교 수의학과
²서울대학교 수의과대학

Abstract

Ninety pigs under the age of 120-day-old requested at the diagnostic laboratory of animal diseases in Cheju National University were evaluated for the prevalence of tissue antigen and serum antibody to swine influenza virus (SIV). For histopathologic examination there was sampled at the consolidated area in cranioventral or dorsocaudal lobes of lungs. Lung tissues from all pigs were tested for the antigen of SIV type A by immunohistochemistry (IHC). Sera from 56 pigs were used for the antibody detection to SIV type A (subtype H1N1 and H3N2) by haemagglutinin inhibition test. Pneumonic lesions were observed in 72 cases (80%) of 90 pigs. Broncho-interstitial or interstitial pneumonia were more prevalent than suppurative or fibrinous bronchopneumonia. According to HI test, 46.4% of the tested sera showed seropositive. Positive sera were consisted with 5.3% for SIV H1N1, 28.6% for SIV H3N2, and 12.5% for both subtype to be tested, respectively. SIV antigens were detected in 51 cases(56.6%) of 90 pigs. Most SIV antigens were presented in the epithelium of the bronchi and bronchiole. Necrotizing bronchitis or bronchiolitis were observed in 28(31.1%) cases of all inspected pigs. These results suggested that SIV might be an important role to induce swine pneumonia in Jeju. Also IHC was very useful for the detection of SIV in the lung.

Key Words: swine influenza virus, pneumonia, immunohistochemistry, haemagglutinin inhibition test