Korean Journal of Veterinary Research 2004;44(4):533-537.
Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP
Tae-Jung Kim1, Myoung-Hee Kim1, Young-Ran Na1, Suk-Chan Jung2, Jae-Il Lee1
1College of Veterinary Medicine, Chonnam National University
2National Veterinary Research and Quarantine Service
NADH oxidase(nox) 유선자의 PCR- RFLP를 이용한 돼지 intestinal spirochetes 국내분리주의 동정
김태중1, 김명희1, 나영란1, 정석찬2, 이재일1
1전남대학교 수의과대학
In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn II. In study using Bfm I, both strains showed no difference in fragmented size(197 and 741 bp). When use Dpn II, B. hyodysenteriae showed two bands(209 and 684 bp), however B. pilosicoli showed a single band of 896 bp. Our results indicate that nox-specific PCR-RFLP could be used as a typing method of Brachyspira species and as an epidemiological method for identifying spirochetes isolated from swine.
Key Words: Brachyspira hyodysenteriae, Brachyspira pilosicoli
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