Korean Journal of Veterinary Research 2004;44(4):539-549.
Comparison of tissue tropism of Newcastle disease vaccine viruses by Immunohistochemistry techniques
Min-Jeong Kim1, Yong-Kuk Kwon1, Hwan-Woo Seong1, Shien-Young Kang2, In-Pil Mo2
1National Veterinary Research and Quarantine Service
2Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University
면역조직화학기법을 이용한 뉴캣슬병 백신바이러스의 조직친화성 비교
김민정1, 권용국1, 성환우1, 강신영2, 모인필2
1국립수의과학검역원
2충북대학교 수의과대학 동물의학연구소
Abstract
Mean death time of inoculated embryonated egg is one of the methods to determine the virulence of the Newcastle disease viruses (NDV). Evaluation of tissue tropism of NDV in the host has been proposed as an another way to determine the pathogenicity of NDV based on the principal site of viral replication. To evaluate the tissue tropism among NDV, an immunohistochemistry(IHC) technique using monoclonal antibody was applied in one-day-old SPF chickens inoculated with different ND vaccine strains such as Ulster 2C, VG/GA and B1 viruses by eye drop instillation. The tissues used for this comparison were trachea, intestine, Harderian gland and cecal tonsil, which were collected at 0.5, 1, 2, 3, 5, 8, 10, 14 days post inoculation. Among test groups, chickens inoculated with B1 viurs, which is known to replicate in the respiratory system, have IHC positive staining mainly in the trachea and those inoculated with Ulster 2C have IHC positive staining mainly in the intestine. However, chickens inoculated with VG/GA strain have IHC positive staining in both the trachea and intestine. Therefore, a differences in tissue tropism among ND vaccine strains has been proved by the IHC technique. Based on this results, the IHC staining technique could be used to classify the NDV or to study the pathogenesis of NDV in chickens.
Key Words: Newcastle disease virus, immunohistochemistry, B1 vaccine strain, Ulter 2C vaccine strain, VG/GA vaccine strain


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