Detection of viral pathogens and isolation of porcine circovirus 2 from postweaning multisystemic wasting syndrome-affected piglets

Park, Choi-Kyu; Kim, Hyun-Soo

Korean Journal of Veterinary Research 2004;44(4):561-569.

Choi-Kyu Park¹, Hyun-Soo Kim²

¹National Veterinary Research and Quarantine Services
²College of Veterinary Medicine, Chungnam National University

이유자돈 전신소모성증후군 이환 자돈에서의 바이러스성 원인체 검색 및 porcine circovirus 2 분리동정

박최규¹, 김현수²

¹국립수의과학검역원
²충남대학교 수의과대학

Abstract

To detect viral agents and isolate porcine circovirus 2 (PCV2), 60 samples of lung and lymph node were collected from 5 to 12 week-old pigs that had showed clinical signs of postweaning multisystemic wasting syndrome (PMWS). Polymerase chain reactions (PCRs) were conducted to identify the viral pathogens including PCV1, PCV2, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) that have been considered to be the causal agents of PMWS. Among 60 samples, PCV 2 was detected from 57 samples but no PCV 1 was detected. PRRSV and/or PPV were also detected from 27 (47.4%) samples and 1 (1.8%) sample of these 57 PCV 2-positive samples, respectively. Tissue homogenates were inoculated onto PCV-free PK-15 cell monolayers. Seven isolates were confirmed as PCV 2 by multiplex PCR, indirect immunofluorescence assay, and transmissible electron microscopy. These date suggest that PRRSV is a major cofactors causing PMWS in pigs that were infected with PCV2 in Korea.

Key Words: postweaning multisystemic wasting syndrome(PMWS), porcine circovirus(PCV), porcine parvovirus( PPV), porcine reproductive and respiratory syndrome virus(PRRSV), polymerase chain reaction(PCR)