Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo |
Eun-Hye Jo1, Sung-Dae Cho1, Nam-Shik Ahn1, Ji-Won Jung1, Se-Ran Yang1, Joon-Suk Park1, Jae-Woong Hwang1, Sung-Hoon Lee1, Jung-Ran Park1, Sun-Jung Kim1, Hyun-Kyung Park2, Yong-Soon Lee1, Kyung-Sun Kang1 |
1Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University 2Sam-Hwa Farm |
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Abstract |
Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo. |
Key Words:
Sargassum fulvellum, MCF-7 cells, apoptosis, Bcl-2 family, xenograft |
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