| The study for the mouse cerebellum developments irradiated with γ-ray during embryogenesis |
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Il-Kwon Park1, Kyoung-Youl Lee1, O-Sung Park1, Sung-Hwa Kim1, Guen-Jwa Lee2, Kang-Yi Lee3, Tae-Sun Min4, Hong-Sun Yook5, Myung-Woo Byun6, Moo-Kang Kim1 |
1College of the Veterinary Medicine, Chung Nam National University
2Chung Nam Veterinary Service Laboratory
3College of Oriental Medicine, Daejeon University
4Department of Life Science, KOSEF
5Department of Food and Nutrition, College of Home Economics
6Korea Atomic Energy Research Institute, Team of Radiation Food and Biotechnology |
| 감마선 조사에 의한 발생과정 중인 마우스의 소뇌발달에 관한 연구 |
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박일권1, 이경열1, 박오성1, 김성화1, 이근좌2, 이강이3, 민태선4, 육홍선5, 변명우6, 김무강1 |
1충남대학교 수의과대학
2충남축산위생연구소
3대전대학교 한의과대학
4한국과학재단
5충남대학교 생활과학대학 식품영양학과
6한국원자력연구소 방사선식품생명공학연구팀 |
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| Abstract |
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In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth. 2. The thickness, proliferation and migration of the 2.5, 5 and 10 Gy irradiated external granular cells from the maginal zone to the medullary area forming the molecular layer from the 6th day to the 15th day after birth were thinner, weaker and more slower according to the radiated dosages than the control group in the cresyl violet staining. 3. The proliteration, migration and lobulation of the 5 Gy radiated groups from the first day to the 15th day after birth were more weak, incomplete and irregular shape in the immunostaining with Dab, Cdk5, P35, calbindin and Zebrin antibody. 4. In the western blotting analysis using the Reelin, Dab, Cdk5 and P35 antibody. The Bands were in the 60 KD, 80 KD, 33 KD and 35 KD, and there were no differences between the control and irradiated groups in the molecular band except the Reelin. 5. As a results, the proliferation and migration of the outer granular and purkinje cells, and lobulation of the cerebellum by the several dosaege of the ${gamma}$-ray radiation were proportionally incomplete according to dosage. |
| Key Words:
$gamma$-ray, development, mouse, cerebellum |
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