Bovine papillomavirus detection from bovine teats using immunohistochemistry and electronmicroscopy |
You-Chan Bae1, Soon-Seek Yoon1, Jung-Won Park1, Cheong-San Lee2, Yong-Hwa Jean1, Mun-Il Kang3 |
1National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry 2Chungbuk Livestock and Veterinary Research Institute 3College of Veterinary Medicine, Chonnam National University |
면역조직화학염색 및 전자현미경검사를 이용한 소 유두로부터 소유두종바이러스 검출 |
배유찬1, 윤순식1, 박중원1, 이청산2, 진영화1, 강문일3 |
1국립수의과학검역원 병리과 2충청북도 축산위생연구소 3전남대학교 수의과대학 |
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Abstract |
We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26. Electronmicroscopically, in Holstein papilloma cases, BPV particles was detected in 31 heads (39.2%) of 79. This study revealed that papilloma was very prevalent in holstein teats, Korea and the prevalence of papilloma in Holstein was 8 times higher than that of Korean native cattle. Moreover, it was needed to consider gross and histopathological lesions to confirm BPV infection on teats because the sensitivity of immunohistochemistry and electronmicroscopy was not high. |
Key Words:
bovine papilloma virus, immunohistochemistry, electronmicroscopy, bovine teat, holstein |
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