| Examinations on sedation, anaesthetic and toxic effect of Isoeugenol for olive-flounder (Paralichthys olivaceus) |
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Gee-wook Shin1, Yong-seung Shin1, Young-rim Kim1, Eun-young Lee1, Hyang-hee Yang1, K.J. Palaksha1, Nam-eung Huh1, Seong-chan Yeon1, Hee-chun Lee1, Myung-ju Oh2, Seong-joon Joh3, Seung-won Kang3, Tae-sung Jung1 |
1Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University
2Department of Fish Pathology, Yosu National University
3National Veterinary Research and Quarantine Service |
| Isoeugenol의 넙치(Paralichthys olivaceus)에 대한 진정, 마취 및 독성효과 검토 |
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신기욱1, 신용승1, 김영림1, 이은영1, 양향희1, 팔락샤1, 허남응1, 연성찬1, 이희천1, 오명주2, 조성준3, 강승원3, 정태성1 |
1경상대학교 수의과대학 동물의학연구소
2여수대학교 수산생명의학과
3국립수의과학검역원 |
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| Abstract |
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Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs & Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and $15^{circ}C$, and 7.5 ppm at $20^{circ}C$, respectively. Anesthesia was required at least 10, 7.5 and 10 ppm at $10^{circ}C$, $15^{circ}C$ and at $20^{circ}C$, respectively. In case of fry, the effect of sedation was observed from 2.5 ppm at $10^{circ}C$ and 5 ppm at 15 and $20^{circ}C$, respectively. Anesthesia was observed from 2.5 ppm at $10^{circ}C$, 5 ppm at 15 and $20^{circ}C$, respectively. In acute toxicity test, it was impossible to explore $LD_{50}$ with the concentration of isoeugenol adult at $15^{circ}C$ used, but over immersion volume of 15 ppm at $15^{circ}C$ was observed mortality in fry. Based on the present study, isoeugenol was identified as a safe and active anesthetic to olive flounder. |
| Key Words:
Isoeugenol, olive-flounder (Paralichthys olivaceus), Anesthetics |
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