| Contamination patterns of Listeria spp. in pork processing plants using random amplified polymorphic DNA |
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Sung-Yeol Ha1, Weon-Sang Choi2, Gyung-Jin Bahk3, Chong-Hae Hong1 |
1Department of Veterinary Medicine, Kangwon National University
2Department of Biotechnology, College of Natural Science, Dongguk University
3HACCP Team, Korea Health Industry Development Institute |
| RAPD를 이용한 돈육 가공장의 Listeria 오염양상 분석 |
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하승열1, 최원상2, 박경진3, 홍종해1 |
1강원대학교 수의학과
2동국대학교 분자생물학과
3한국보건산업진흥원 |
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| Abstract |
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This study was carried out to understand the contamination patterns of Listeria in pork processing plants. A total of 402 samples were collected from carcass, pork during processing, surfaces of equipment and environment, and 238 isolates of Listeria species were identified. L. innocua was found in 64.7% of the isolates, L. monocytogenes in 33.2%, and L. welshimeri in 2.1%. Random amplified polymorphic DNA (RAPD) analysis performed to investigate the origin and routes of Listeria contamination, showed 21 composite types of L. monocytogenes and 26 composite types of L. innocua. It was confirmed that Listeria contamination begins with contaminated incoming carcass and ever-present contaminants in the processing environments. The persistence and dissemination of the same strain of L. monocytogenes and L. innocua throughout the processing line revealed that the sanitation standard operating procedure should be implemented to minimize the risk of colonization in the workplace. Molecular subtyping of L. innocua allowed us to tracing the possibility of cross-contamination during processing. |
| Key Words:
Pork processing plant, Listeria monocytogenes, Listeria innocua, RAPD |
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