| Expression of caveolin-3 as positive intracellular signaling regulator on the development of hypertrophy in cardiac tissues |
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Joo-Heon Kim1, Jin Han2, Yong-Kwon Kim3, Young-Ae Yang3, Yonggeun Hong2 |
1Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University
2Cardiovascular & Metabolic Disease Center, College of Medicine, Inje University
3College of Biomedical Science and Engineering, Inje University |
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| Abstract |
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We have examined distribution and expression of caveolin-3 (cav-3), one of three caveolin isoforms from 16-wks-old spontaneously hypertensive rats (SHR) compared with age-matched control wistar-kyoto (WKY) rats. The expression of cav-3 was increased, whereas expression of PKB/Akt and calcineurin (Cn) was not changed in cardiac tissues of SHR compared to WKY rats. Interestingly, expression of cav-3, PKB/Akt and Cn were decreased in plasma membrane fraction in SHR compared to WKY rats. In H9c2 cardiomyoblast cells treated with phenylephrine ($50{mu}M$, 48hr) or isoproterenol ($10{mu}M$, 48hr), the expression of cav-3 was markedly enhanced compared to nontreated cells. Upon immunofluorescence analysis, cav-3 was localized in plasma membrane of control H9c2 cells. However phenylephrine or isoproterenol treatment caused translocation of cav-3 to perinuclear region. These results suggest that cav-3 plays as positive regulators in the development of hypertrophy in cardiac tissues of SHR rats. |
| Key Words:
calcineurin, caveolin, hypertrophy, SHR, spontaneously hypertensive rat, PKB/Akt |
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