Korean Journal of Veterinary Research 2005;45(4):545-552.
Estrogenic Effects of endocrine disruptors and establishment of screening methods in mice
Ji-Youn Jung, Yong-Soon Lee
College of Veterinary Medicine, Seoul National University
실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구
정지윤, 이영순
서울대학교 수의과대학
Abstract
The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{eta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{eta}$-Bestradiol and nonylphenol, $17{eta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.
Key Words: di-ethylhexyl phthalate, di-n-butyl phthalate, endocrine disruptors, estrogenic effects


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