Over-expression of Chlamydia psittaci MOMP in Escherichia coli and its purification

Ha, Jung-Soon; Lee, Do-Bu; Han, Sang-Hoon; Lim, Yoon-Kyu; Yoon, Byoung-Su

Korean Journal of Veterinary Research 2006;46(1):13-19.

Jung-Soon Ha¹, Do-Bu Lee¹, Sang-Hoon Han¹, Yoon-Kyu Lim², Byoung-Su Yoon¹

¹Department of Biology, Kyonggi university
²Department of Veterinary medicine, Cheju National University

대장균에서 Chlamydia psittaci MOMP 유전자의 과발현과 순수분리

하정순¹, 이도부¹, 한상훈¹, 임윤규², 윤병수¹

¹경기대학교 생물학과
²제주대학교 수의학과

Abstract

Generally known psittacosis or ornithosis is a disease of birds caused by the bacterium Chlamydia psittaci. Humans are accidential hosts and are most commonly infected from avian sources. It raises hepatitis or neurosis. As major outer membrane protein (MOMP) of Chlamydia psittaci has been known to play a role in the avoidance of host immune defenses, research on developing a Chlamydia vaccine has focused on the MOMP. In this study, the gene encoding the major outer membrane protein (MOMP) of the Chlamydia psittaci strain 6BC was cloned and expressed in Escherichia coli strain M-15. The recombinant DNA was cloned by fusion prokaryotic expression vector pQE30-GFPII. Expression of the recombinant protein was performed in E. coli and was induced by IPTG. The size of expressed recombinant protein is 74.220 kDa (MOMP, 43.260 kDa; GFP expression region, 30 kDa; $6{ imes}His$ tag, 960Da). This protein was purified by using his-tagging-inclusion body. Recombinant protein was reconfirmed through ELISA test and western blot with antibody against pQE30-GFPII. It will be useful antibody development.

Key Words: Chlamydia psittaci, Major outer membrane protein, pQE30-GFPII