Korean Journal of Veterinary Research 2006;46(1):13-19.
Over-expression of Chlamydia psittaci MOMP in Escherichia coli and its purification
Jung-Soon Ha1, Do-Bu Lee1, Sang-Hoon Han1, Yoon-Kyu Lim2, Byoung-Su Yoon1
1Department of Biology, Kyonggi university
2Department of Veterinary medicine, Cheju National University
대장균에서 Chlamydia psittaci MOMP 유전자의 과발현과 순수분리
하정순1, 이도부1, 한상훈1, 임윤규2, 윤병수1
1경기대학교 생물학과
2제주대학교 수의학과
Generally known psittacosis or ornithosis is a disease of birds caused by the bacterium Chlamydia psittaci. Humans are accidential hosts and are most commonly infected from avian sources. It raises hepatitis or neurosis. As major outer membrane protein (MOMP) of Chlamydia psittaci has been known to play a role in the avoidance of host immune defenses, research on developing a Chlamydia vaccine has focused on the MOMP. In this study, the gene encoding the major outer membrane protein (MOMP) of the Chlamydia psittaci strain 6BC was cloned and expressed in Escherichia coli strain M-15. The recombinant DNA was cloned by fusion prokaryotic expression vector pQE30-GFPII. Expression of the recombinant protein was performed in E. coli and was induced by IPTG. The size of expressed recombinant protein is 74.220 kDa (MOMP, 43.260 kDa; GFP expression region, 30 kDa; $6{ imes}His$ tag, 960Da). This protein was purified by using his-tagging-inclusion body. Recombinant protein was reconfirmed through ELISA test and western blot with antibody against pQE30-GFPII. It will be useful antibody development.
Key Words: Chlamydia psittaci, Major outer membrane protein, pQE30-GFPII
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