Korean Journal of Veterinary Research 2006;46(1):21-25.
Iridovirus infection of cultured juvenile flounder (Paralichthys olivaceus) in nursery
Tae-Jung Kim1, Eun-Jin Jang1, Jong-Su Kim2, Jae-Il Lee1
1College of Veterinary Medicine, Chonnam National University
2Jeonnam Biotechnology Research Center
종묘장 넙치 치어에서의 Iridovirus 감염
김태중1, 장은진1, 김종수2, 이재일1
1전남대학교 수의과대학
2전남생물산업연구센터
Abstract
Iridovirus is an icosahedral cytoplasmic double-stranded DNA virus with a genome size of 170-200kb. Outbreaks of fish iridovirus infection are characterized by their wide geographic distribution and broad host spectrum, especially in water temperatures of $25-27^{circ}C$ Recently, the causative agent of high mortalities in flounder (Paralichthys olivaceus) was identified as fish iridovirus in Korea. Iridoviral infection repeatedly occurs in the same area for long periods, suggesting the possibility of viral infection in nursery. To examine this, the existence of iridovirus in juvenile flounders was detected by PCR using virus-specific primers. Antibodies induced against this virus were also examined using ELISA. As a result, juvenile fish in nursery were found to be previously infected with iridovirus, suggesting that proper prevention systems are required.
Key Words: ELISA, iridovirus, PCR, recombinant protein


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