Korean Journal of Veterinary Research 2006;46(1):35-41.
An in vitro vitellogenin bioassay for estrogenic substances in the Rhynchocypris oxycephalus
Jung-A Shin1, Jae-Young Song1, Hee-Young Lim1, Min-Ho Chang1, Hye-Min Lee1, Kyu-Hoi Chung1, Yoon-Kyu Lim2, Byoung-Su Yoon1
1Department of Biology, Kyonggi University
2Department of Veteinary medicine, Cheju National University
버들치(Rhynchocypris oxycephalus)에서 에스트로겐류 물질에 의한 vitellogenin의 유도 및 단일클론항체 제작
신정아1, 송재영1, 임희영1, 장민호1, 이혜민1, 정규희1, 임윤규2, 윤병수1
1경기대학교 생물학과
2제주대학교 수의학과
Abstract
Vitellogenin (Vtg), a phospholipoglycoprotein precursor of egg yolk is synthesized and secreted from the liver in response to estrogens in female fish. Vtg is normally undetectable in the blood of male fish, but can be induced by exposure to chemicals possessing estrogenic activity. Thus, the presence of Vtg in blood of male fish can serve as a useful biomarker for assessing previous exposure to estrogenic compounds. In the present study, Vtg was abnormally expressed in Rhynchocypris oxycephalus using estradiol benzoate ($E_2$). As the result, it was found that the level of Vtg in blood from R. oxycephalus was increased by treated quantity of $E_2$ with dose-effect manner. Monoclonal antibodies were generated against Vtg of R. oxycephalus. The hybridoma were screened with an enzyme immunoassay for the production of specific anti-Vtg antibodies. Five positive cell lines with a high specificity were selected. Monoclonal antibodies against vtg of R. oxycephalus that was developed in this study, may be a useful bio-indicator for the detection of estrogenic contamination in the aquatic ecosystem.
Key Words: bio-marker, estrogenic compound, monoclonal antibody, R. oxycephalus, vitellogenin
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