Evaluation of the extract from lymph nodes for Salmonella monitoring in pig herds

Jung, Byeong-Yeal; Choo, Ji-Hoon; Kim, Ji-Hun; Jung, Jae-Yun

Korean Journal of Veterinary Research 2006;46(2):119-125.

Byeong-Yeal Jung¹, Ji-Hoon Choo², Ji-Hun Kim², Jae-Yun Jung²

¹National Veterinary Research and Quarantine Service
²College of Veterinary Medicine, Kyungpook National University

돈군의 Salmonella 모니터링을 위한 림프절 추출액 사용에 대한 평가

정병열¹, 추지훈², 김지훈², 정재윤²

¹국립수의과학검역원
²경북대학교 수의과대학

Abstract

The objective of this study was to investigate the use of extract from mesenteric lymph nodes as an alternative to serum for ELISA to detect Salmonella antibodies in slaughter pigs. Among 324 slaughter pigs, 65 (20.1 %) were positive in the serum ELISA and 76 (23.5%) were positive in the ELISA with extract from lymph nodes. A total of 24 (7.4%) Salmonella representing 6 serotypes were isolated from mesenteric lymph nodes and 35 (10.8%) Salmonella belonging to 2 serotypes were also recovered from cecal contents of slaughter pig samples, respectively. The most prevalent serogroup was B (55.9% of isolates) and serotype was Typhimurium (52.5% of isolates). In the comparison of the results of between the serum ELISA and Salmonella isolation, kappa value was 0.28 with mesenteric lymph nodes and 0.37 with cecal contents, respectively. However, the extract ELISA had sensitivity of 98.5%, specificity of 95.4% and kappa value of 0.88 as compared with the serum ELISA. Because high degree of concordance between the serum ELISA and the extract ELISA was observed (P=0.24), extract from lymph nodes could be used as an alternative to serum for the detection of Salmonella antibodies in the ELISA.

Key Words: ELISA, extract of lymph nodes, Salmonella, serum, slaughter pigs