Korean Journal of Veterinary Research 2006;46(2):119-125.
Evaluation of the extract from lymph nodes for Salmonella monitoring in pig herds
Byeong-Yeal Jung1, Ji-Hoon Choo2, Ji-Hun Kim2, Jae-Yun Jung2
1National Veterinary Research and Quarantine Service
2College of Veterinary Medicine, Kyungpook National University
돈군의 Salmonella 모니터링을 위한 림프절 추출액 사용에 대한 평가
정병열1, 추지훈2, 김지훈2, 정재윤2
1국립수의과학검역원
2경북대학교 수의과대학
Abstract
The objective of this study was to investigate the use of extract from mesenteric lymph nodes as an alternative to serum for ELISA to detect Salmonella antibodies in slaughter pigs. Among 324 slaughter pigs, 65 (20.1 %) were positive in the serum ELISA and 76 (23.5%) were positive in the ELISA with extract from lymph nodes. A total of 24 (7.4%) Salmonella representing 6 serotypes were isolated from mesenteric lymph nodes and 35 (10.8%) Salmonella belonging to 2 serotypes were also recovered from cecal contents of slaughter pig samples, respectively. The most prevalent serogroup was B (55.9% of isolates) and serotype was Typhimurium (52.5% of isolates). In the comparison of the results of between the serum ELISA and Salmonella isolation, kappa value was 0.28 with mesenteric lymph nodes and 0.37 with cecal contents, respectively. However, the extract ELISA had sensitivity of 98.5%, specificity of 95.4% and kappa value of 0.88 as compared with the serum ELISA. Because high degree of concordance between the serum ELISA and the extract ELISA was observed (P=0.24), extract from lymph nodes could be used as an alternative to serum for the detection of Salmonella antibodies in the ELISA.
Key Words: ELISA, extract of lymph nodes, Salmonella, serum, slaughter pigs


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