Korean Journal of Veterinary Research 2006;46(2):135-142.
Identification and characterization of Shiga toxin-producing Escherichia coli isolated from diarrhea in calves
Keum-Gi Lim, Mun-Il Kang, Snag-Ki Kim, Kyung-Woo Nam, Hyun-Joo Park, Jin- Ryang Park, Kyoung-Oh Cho, Bong-Joo Lee
College of Veterinary Medicin, Chonnam National University
송아지 설사분변으로부터 Shiga toxin-producing Escherichia coli 의 분리 및 특성규명
임금기, 강문일, 김상기, 남경우, 박현주, 박진량, 조경오, 이봉주
전남대학교 수의과대학
Abstract
Shiga toxin (stx) producing Escherichia coli (STEC) causes various clinical signs in animal and human. In this study, 255 fecal samples from calves showing diarrhea were collected from cattle farms in Chonnam province during the period from January 2005 to July 2005. Twenty six STEC (10%) were isolated from 255 fecal samples by PCR. The isolates displayed three different stx combinations (stx1 [69%], stx1 and stx2 [15%], and stx2 [38%]). The isolates were further studied for virulence associated genes and antimicrobial resistance to define the virulence properties. Intimin (eaeA), enterohemolysin (hlyA), and lipopolysaccharide (rfbE) virulence genes were detected in 6 (23%), 7 (26%), and 1 (3.8%) of the isolates, respectively, by PCR. One isolate possessing rfbE gene was typed as E. coli 0157 : H7 by agglutination test with O and H antisera. All 26 isolates showed susceptibility to amikacin (100%) and the majority of isolates showed high susceptibility to gentamicin (88.5%) and chloramphenicol (73.1%). But all isolates were resistant to penicillin. These results may provide the basic knowledge to establish strategies for the treatment and prevention of enteric disease in calves.
Key Words: antimicrobial resistances, calves, fecal samples, STEC, stx gene


About
Browse articles
For contributors
Policy
Editorial Office
#401-1, 85 Bldg., College of Veterinary Medicine, Seoul National University
1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea
Tel: +82-2-880-1229    Fax: +82-2-878-9762    E-mail: jvs@ksvs.or.kr                

Copyright © 2024 by The Korean Society of Veterinary Science.

Developed in M2PI

Close layer
prev next