Korean Journal of Veterinary Research 2006;46(4):305-313.
Silk fibroin/hyaluronic acid blend sponge accelerates the wound healing in full-thickness skin injury model of rat
Seuk-Yun Kang1, Dae-Hyun Roh1, Hyun-Woo Kim1, Seo-Yeon Yoon1, Young-Bae Kwon2, HaeYong Kweon3, Kwang-Gill Lee3, Young-Hwan Park4, Jang-Hern Lee1
1College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University
2Institute for Medical Science, Chonbuk National University Medical School
3National Institute of Agricultural Science and Technology
4College of Agriculture and Life Sciences, Seoul National University
전층피부창상에서 실크피브로인과 하이알론산 혼합 스폰지의 창상치유효과
강석윤1, 노대현1, 김현우1, 윤서연1, 권영배2, 권해용3, 이광길3, 박영환4, 이장헌1
1서울대학교 수의과대학
2전북대학교 의과대학
3국립농업과학진흥청 잠사연구소
4서울대학교 농업생명과학대학
The primary goal of the wound healing is rapid wound closure. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biologic processes involved in wound repair and tissue regeneration. This study was conducted to develop a new sponge type of biomaterial to be used for either wound dressing or scaffold for tissue engineering. We designed to make a comparative study of the wound healing effect of silk fibroin/hyaluronic acid (SF/HA) blend sponge in full-thickness dermal injury model of rat. Two full-thickness excisions were made on the back of the experimental animals. The excised wound was covered with either the silk fibroin (SF), hyaluronic acid (HA) or SF/HA (7 : 3 or 5 : 5 ratio) blend sponge. On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. Simultaneously, the tissues were stained with Hematoxylin-Eosin and Masson's trichrome methods to measure the area of regenerated epithelium and collagen deposition. In addition, we evaluated the degree of the epithelial cell proliferation using immunohistochemistry for proliferating cell nuclear antigen (PCNA). We found that the half healing time ($HT_{50}$) of SF/HA blend sponge treated groups were significantly decreased as compared with either those of SF or HA treatment group. Furthermore, SF/HA blend sponges significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as compared with those of control treatment. Especially, the 5 : 5 ratio group of SF/HA among all treatment groups was most effective on wound healing rate and histological studies. These results suggest that SF/HA blend sponges could accelerate the wound healing process through the increase of epithelialization, collagen deposition and basal cell proliferation in full thickness skin injury.
Key Words: epithelialization, hyaluronic acid, proliferating cell nuclear antigen, silk fibroin, wound healing
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