| Immunogenicity of the recombinant Pasteurella multocida toxin for development of subunit vaccine against swine atrophic rhinitis |
|
Jeongmin Lee |
|
Institute of Life Science and Technology, Sungkyunkwan University |
| 돼지 위축성 비염 단위 백신 개발을 위한 재조합 파스튜렐라 독소 단백질의 면역원성 검정 |
|
이정민 |
|
성균관대학교 생명공학연구소 |
|
|
| Abstract |
|
Pasteurella multocida Pasteurella multocida toxin (PMT) is a causal pathogenin atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinantPMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment(PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system.The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-afinitycolumn chromatography. The PMT2.3 raised slightly less anti-PMT antibody titer than formalin-killedwhole cel, however, it showed more protective imunity against P. multocida D:4 infection in vaccinationand chalenge. |
| Key Words:
atrophic rhinitis, immunogenicity, Pasteurella multocida toxin, subunit vaccine |
|
PDF Links
Full text via DOI
Download Citation
