Korean Journal of Veterinary Research 2007;47(1):67-75.
Antimicrobial susceptibility and prevalence of gram-negative bacteria isolated from bovine mastitis
Eun-Sil Lee1, Hyun-Mi Kang2, Chung-il Chung1, Jin-San Moon2
1Department of Dairy Science, Konkuk University
2National Veterinary Research and Quarantine Service
젖소 유방염으로부터 분리한 그람음성균의 분포 및 항생제 감수성
이은실1, 강현미2, 정충일1, 문진산2
1건국대학교 낙농학과
Environmental mastitis has increased particularly in well-managed or low somatic cell countherds that have successfully controled contagious pathogens. Major pathogens of environmental mastitisare Escherichia coli (E. coli) and Streptococcus uberis. The present study was conducted to investigate1,865 quaters of 241 Korean dairy farms from 2001 to 2004. Prevalence of major gram-negative bacteriaisolated from mastitis milk were E. coli (22.7%) and Enterobacter spp. (16.3%) in coliforms and Pseudomoassp. (10.3%) and Serratia spp. (7.9%) in non-coliforms. The results on antibiotic susceptibility by agardifusion test against these pathogens were 86.7% in piperaciliin, 94.6% in cefepime, 85.5% in amikacin,87.7% in gentamicin and so on. In contrast, the susceptibility against ampicillin (41.9%), cephalothin (9.9%),streptomycin (39.9%) and tetracycline (46.7%) appeared to be below 50%. Gram-negative bacteria showed(96.8%). Acording to year, distribution of high $256{sim}64{mu}g/ml$ on cephalothin get increased, but the othersare diferent. These findings demonstrate that major gram-negative bacteria were E. coli and Enterobacterspp. isolates, and often encountered the diverse antibiotic resistant patterns.
Key Words: antimicrobial susceptibility, cow, gram negative bacteria, mastitis

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