| Successful pregnancy following surgical intrauterine insemination using frozen semen in Afghan hound dog |
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Hye Jin Kim1, Hyun Ju Oh1, Goo Jang1, Jung Hee Yoon1, Hyung Suk Lee2, Sang Cheol Kim3, Cheol Young Hwang1, Dae Yong Kim1, Min Kyu Kim1 |
1College of Veterinary Medicine, Seoul National University
2Woosong Information College
3Department of Animal Science, Shingu College |
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| Abstract |
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A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was $59.4{ imes}10^6$and the total volume was 1.2 ml. The frozen spermatozoa were thawed in $70^{circ}C$ water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method. The number of corpus luteum was 6. Sixty days after artificial insemination resulted in the birth of 4 puppies, all of which are alive and healthy. |
| Key Words:
artificial insemination, frozen dog semen, intrauterine insemination |
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