Estimation of uncertainty for the determination of residual flubendazole in pork

Kim, MeeKyung; Park, Su-Jeong; Lim, Chae-Mi; Cho, Byung-Hoon; Kwon, Hyun-Jeong; Kim, Dong-Gyu; Chung, Gab-Soo

Korean Journal of Veterinary Research 2007;47(2):139-145.

MeeKyung Kim, Su-Jeong Park, Chae-Mi Lim, Byung-Hoon Cho, Hyun-Jeong Kwon, Dong-Gyu Kim, Gab-Soo Chung

National Veterinary Research and Quarantine Service

돼지고기 중 플루벤다졸 잔류분석의 불확도 추정

김미경, 박수정, 임채미, 조병훈, 권현정, 김동규, 정갑수

국립수의과학검역원

Abstract

Measurement uncertainty could play an important role in the assessment of test results in laboratories and industries. We investigated measurement uncertainties possibly included in determination of flubendazole, a benzimidazole anthelmintic, in pork by HPLC. The concentration of flubendazole was 62.69 ng/g in a sample of pork. Uncertainty was estimated in the analytical procedure of flubendazole. A model equation was made for determination of flubendazole in pork. The four uncertainty components such as weight of sample, volume of sample, calibration curve, and recovery were selected to estimate measurement uncertainties. Standard uncertainty was calculated for each component and all the standard uncertainties were combined. The combined standard uncertainty was expanded to a sample population as an expanded uncertainty. The expanded uncertainty was calculated using k value on Student's t-table and effective degrees of freedom from Welch-Satterthwaite formula. The expanded uncertainty was calculated as 3.45 with the combined standard uncertainty, 1.584 6 and the k value, 2.18. The final expression can be ($62.69{pm}3.45$) ng/g (confidence level 95%, k = 2.18). The uncertainty value might be estimated differently depending on the selection of the uncertainty components. It is difficult to estimate all the uncertainty factors. Therefore, it is better to take several big effecting components instead of many small effecting components.

Key Words: flubendazole, HPLC, pork, uncertainty, veterinary drugs