Korean Journal of Veterinary Research 2007;47(2):163-167.
Prevalence for persistent infection with bovine viral diarrhea virus in Korean native calves
You-Chan Bae, Ha-Young Kim, Jung-Won Park, Soon-Seek Yoon, Gye-Hyeong Woo, O-Soo Lee, Mun-Il Kang
National Veterinary Research and Quarantine Service
한우 송아지의 소바이러스성 설사바이러스 지속감염률 조사
배유찬, 김하영, 박중원, 윤순식, 우계형, 이오수, 강문일
Bovine viral diarrhea (BVD) is very important disease in cattle industry with a worldwide distribution. Detection and elimination of persistently infected calves with bovine viral diarrhea virus (BVDV) was valuable strategy for BVD eradication because those calves were main source for transmission. We surveyed persistent infection with BVDV by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) using whole blood and skin. Five hundred thirty nine Korean native calves were tested. Four calves (0.7%) were positive for BVDV antigen for both tests. Those calves remained positive for follow-up by RT-PCR and IHC. Therefore they were identified as persistently infected with BVDV. We confirmed that immunohistochemistry using skin biopsy samples was very useful tool to detect persistently infected calves with BVDV. As far as we know, this would be first report on persistent infection with BVDV in Korea.
Key Words: bovine viral diarrhea virus, immunohistochemistry, Korean native calf, persistent infection

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