| Microwave fixation of Setaria Digitata for scanning electron microscopy |
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San-Soo Lee1, Kyoung-Oh Cho1, Kil-Sang Shin2, Sung-Shik Shin1 |
1Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University
2College of Natural Sciences, Soonchunhyang University |
| 선충류의 주사전자현미경적 관찰을 위한 마이크로웨이브 고정법 |
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이산수1, 조경오1, 신길상2, 신성식1 |
1전남대학교 수의과대학
2순천향대학교 자연과학대학 |
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| Abstract |
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Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens. |
| Key Words:
microwave fixation, nematode, Setaria digitata |
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