Korean Journal of Veterinary Research 2007;47(2):219-228.
Comparison of osteoinductive efficacy of freezing, freeze-drying and defat-freezing implant preparation for allograft in rabbit
Jong-Il Lee1, Ha-Na Song2, Nam-soo Kim3, In-hyuk Choi3
1Graduate School of Agricultural and Life Sciences, The University of Tokyo
2Seosin Animal Hospital
3College of Veterinary Medicine, Chonbuk National University
토끼 동종골이식에서 이식골편의 동결법, 동결건조법, 탈지 후 동결법 처리에 따른 신생골 형성능력 비교
이종일1, 송하나2, 김남수3, 최인혁3
1동경대학교 농학생명과학대학원
2전주 서신동물병원
3전북대학교 수의과대학
Bone allograft had been increased the need because autogenous bone graft is associated with donor site morbidity and is restricted in quantity. The bone allograft implants have to prepare properly for increasing osteoinductive ability and decreasing immune responses before providing to graft. The purpose of this study was to investigate the efficacy on new bone formation in bone allografts by treatment of implants. Cortical bone allografts were transplanted to experimental defects on midshaft of diaphyseal fibulae in 15 rabbits, which were divided to 3 experimental groups according to the preparation methods-freezing, freeze-drying, defat-freezing. The grafted regions of fibulae of all groups had been radiographed biweekly for 16 weeks to observe new bone formation and union between implant and recipient bone. Bone Alkaline Phosphatase (BALP) in all groups was evaluated biweekly till the end of the experiment to determine osteoblast activities. Unions between implant and recipient bone were observed at 30% (3 of 10 cases) of freezing, 50% (5 of 10 cases) of freeze-drying and 80% (8 of 10 cases) of defat-freezing. BALP was increased over 100% from before graft at 2 weeks of graft procedures in all union cases of freezing and defat-freezing group, then gradually decreased till 16th week. In non-union cases, there is no significant variation in BALP value. Defat-freezing method for allograft implants might be more effective for osteoinductive efficacy of implants than freezing and freeze-drying method.
Key Words: allograft, BALP, defat-freezing, freeze-drying, freezing
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