Korean Journal of Veterinary Research 2007;47(4):389-393.
Occurrence of aflatoxin $M_1$ in milk determined by HPLC with derivatization method in Korea (1999-2000)
Hwan Goo Kang, Joon Hyoung Cho
National Veterinary Research and Quarantine Service
Abstract
In this study, the levels of aflatoxin $M_1$ ($AFM_1$) in milk were determined by HPLC with derivatization method. Milk samples were purified using $C_{18}$ disposable cartridge followed by derivatization with trifluoroacetic acid and analysed using HPLC with fluorescence detection. The recoveries of AFM1 from milk samples added $AFM_1$ at a level of 0.025~0.1 ng/ml were 94.7~98.0% with detection limit of 0.009 ng/ml. The amounts of $AFM_1$ were determined below 0.05 ng/ml for all tested samples of commercial milk collected in 1999 and 2000.
Key Words: Aflatoxin $M_1$, HPLC, milk
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