| Determination of eugenol in Eugenia caryophyllata by high-performance Liquid chromatography with photodiode array detection and method validation |
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Hyeong-Jun Yun, So-Mi Yun, Myoung-Heon Lee, Seong-Wan Son |
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National Veterinary Research and Quarantine Service |
| HPLC-PDA를 이용한 정향(Eugenia caryophyllata) 중의 eugenol 분석법 확립 및 검증 |
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윤형준, 윤소미, 이명헌, 손성완 |
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국립수의과학검역원 |
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| Abstract |
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A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at $60^{circ}C$ for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $0.0125~1{mu}g/ml$. The limit of detection per sample injection ($20{mu}l$) was $0.81ng/{mu}l$ and limit of quantification was $2.47ng/{mu}l$. The method showed good intra-day precision (%RSD 0.08 ~ 0.27%) and inter-day precision (%RSD 0.32 ~ 1.19%). |
| Key Words:
clove, eugenol, HPLC, validation |
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