Korean Journal of Veterinary Research 2008;48(1):33-38.
Determination of amitraz by high-performance Liquid chromatography with photodiode array detection and method validation
Hyeong-Jun Yun, So-Mi Yun, Myoung-Heon Lee, Seong-Wan Son
National Veterinary Research and Quarantine Service
HPLC-PDA를 이용한 Amitraz의 분석법 확립 및 검증
윤형준, 윤소미, 이명헌, 손성완
국립수의과학검역원
Abstract
In veterinary medicine amitraz has been used as an insecticide to eliminates mites, lice, and ticks in dogs, cats, goats, swine and cattle. The objective of present study was to developed an analytical method using one-step extraction and determination of the amitraz in veterinary drugs by liquid chromatography (LC). The amitraz was analyzed by LC equipped with Waters XTerra RP18 ($4.8{ imes}250mm;;5{mu}m;;Waters,;USA$) analytical column, using 75% acetonitrile (acetonitrile/D.W; 75/25) at 1.0 ml/ min. The UV-VIS detection of amitraz was made at 290 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $80{sim}120{mu}g/ml$. The limit of detection was $0.09{mu}g/ml $ and limit of quantification was $0.27{mu}g/ml $. The method showed good intra-day precision (CV 0.05~0.09%) and inter-day precision (CV 0.06~0.18%).
Key Words: amitraz, LC, veterinary drug


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