| Field efficacy of a combined vaccine supplemented with recombinant Pasteurella multocida toxin subunits against atrophic rhinitis |
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Mi Lan Kang1, Seung Won Shin1, Nabin Rayamahji1, Yeon Soo Seo1, Su In Lee1, Won Hyung Lee2, Han Sang Yoo1 |
1College of Veterinary Medicine, BK21 for Veterinary Science and KRF Zoonotic Disease Priority Research Institute, Seoul National University
2XP Bio Inc |
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| Abstract |
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We have investigated efficiency of a recombinant subunit Pasteurella multocida toxin (PMT) that was mixed with a vaccine consisted of inactivated whole cells of Bordetella bronchiseptica, P. multocida (types A and D). For verification of the efficacy of the vaccine, all experimental pigs (suckling piglets, sow and gilts) in the three farms were vaccinated. Antibody titers against B. bronchiseptica and P. multocida type A of the vaccinated pigs by microplate agglutination were significantly higher than those of the control pigs (p < 0.05). Similar patterns were observed in the analysis of anti- PMT neutralizing antibody by serum neutralizing method using Vero cell (p < 0.05). Anti- P. multocida type D antibody titer of the vaccinated sows and gilts by ELISA showed significant differences with those of the non-vaccinated pigs (p < 0.05). Although antibody titers increased, it was unable to find out the difference in the clinical signs between the vaccinated and non-vaccinated pigs. However, the increase in body weight of the vaccinated piglets was observed in comparison with the non-vaccinated piglets on a farm. At slaughtering of the pigs, pathological lesions in the turbinate bones of the vaccinated pigs were significantly lower than those of the non-vaccinated pigs (p < 0.001). These results suggested that efficacy of the vaccine in pigs demonstrated to protect against atrophic rhinitis in Korea. |
| Key Words:
atrophic rhinitis, field trial, Pasteurella multocida toxin, recombinant subunit vaccine |
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