Prevalence and genotypes of pestivirus in Korean goats

Yang, Dong-Kun; Kweon, Chang-Hee; Kim, Byoung-Han; Choi, Cheong-Up; Kang, Mun-Il; Hyun, Bang-Hun; Hwang, In-Jin; Lee, Cheong-San; Cho, Kyoung-Oh

Korean Journal of Veterinary Research 2008;48(1):83-88.

Dong-Kun Yang¹, Chang-Hee Kweon¹, Byoung-Han Kim¹, Cheong-Up Choi¹, Mun-Il Kang¹, Bang-Hun Hyun¹, In-Jin Hwang¹, Cheong-San Lee², Kyoung-Oh Cho³

¹National Veterinary Research and Quarantine Service
²Chungbuk Livestock and Veterinary Research Institute
³Chonnam National University

Abstract

In total, 1,142 serum samples were collected from 223 goat flocks rising in five different regions of Korea. These samples were screened for the presence of border disease virus (BDV) antibodies using an enzyme linked immunosorbent assay. Of the 1,142 samples, we found 47 bovine viral diarrhea virus (BVDV) positive cases (4.1%). These positive serum samples were also examined further by using the virus neutralization test against BDV. In addition, samples were tested for both BVDV and classical swine fever virus (CSFV). All of the samples that were seropositive for BDV also demonstrated positive antibody titers against BVDV and CSFV. Due to their common antigenicity, we also determined further the prevalence and carried out virus neutralization test against three pestiviruses: 314 of the goat samples were screened using reverse transcription polymerase chain reaction with primer pairs specific to common pestivirus genome regions. Overall, 1.6% (5/314) of the samples tested was positive for pestivirus. Based on the nucleotide sequence data and the phylogenetic analysis, three isolates were characterized as BVDV type 1 and two isolates as BVDV type 2. However, none of the isolates could be classified as BDV. These results indicate that BVDV-1 and BVDV-2 are the pestivirus strains circulating among Korean goat populations.

Key Words: genotype, goat, pestivirus, RT-PCR