Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea |
Seong-Joon Joh1, Youn-Jeong Lee1, Chang-Sun Song2, Shien-Young Kang3, In-Pil Mo3, Gang-Jun Heo3 |
1National Veterinary Research and Quarantine Service 2College of Veterinary, Konkuk University 3College of Veterinary Medicine, Research Institute of Veterinary Medicine, Chungbuk National University |
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Abstract |
The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae. |
Key Words:
marine aquatic birnavirus(MABV), phylogenetic relationship, rockfish Sebastes schlegeli, VP2-NS-VP3 |
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