Inhibitory effects of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa) on larval migration of Anisakis spp. in vitro

Kwon, Hee-Nyung; Jee, Cha-Ho

Korean Journal of Veterinary Research 2008;48(4):473-480.

Hee-Nyung Kwon, Cha-Ho Jee

College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University

시험관내에서 아니사키스 유충의 운동성에 대한 고련피, 관중, 사군자의 억제효과

권희녕, 지차호

충북대학교 수의과대학 및 동물의학연구소

Abstract

A high incidence of Anisakiasis has been reported in many countries where people eat frequently raw or undercooked seafood. Anisakis spp. larvae were obtained from the mackerel acquired from a fish market of Cheongju city. They were divided into several groups and placed in culture dishes containing RPMI-1640 (culture media), in the presence or absence of different concentrations of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa). The objective of the present study was to investigate the activity of larval migration inhibition in vitro. Meliae ezadarach at the concentrations of 7.5, 15, and 30 mg/ml effectively inhibited the larvae migration in time-dependent manner during experimental period of 0-24 h. Treatment of Meliae ezadarach at the three concentrations completely inhibited the larvae migration in vitro. Dryopteris crassirhizoma at the concentrations of 5, 10, and 20 mg/ml also effectively inhibited the larvae migration in a time-dependent manner. The treatment of Dryopteris crassirhizoma for 12 h completely inhibited the larvae migration. The inhibitory effect of Dryopteris crassirhizoma was stronger than that of Meliae ezadarach. Although Quisqualis indica var villosa also showed the inhibitory effect on larvae migration, its inhibitory efficacy was the weakest among tested herbal extracts. These results indicated that some herbal extracts may be useful in controlling human anisakiasis.

Key Words: Anisakiasis, Dryopteris crassirhizoma, larval migration inhibition, Meliae ezadarach, Quisqualis indica var villosa