Korean Journal of Veterinary Research 2009;49(1):45-57.
Characterization of the genomes of Aujeszky's disease virus isolated in Korea
Bang-Hun Hyun1, In-Joong Kim1, Hyun-Mi Pyo1, Sang-Ho Cha1, Ji-Yeun Park1, Jae-Young Song1, In-Soo Cho1, Chang-Bum Yang1, Soo-Hwan An1, Joong-Bok Lee2
1National Veterinary Research Institute, National Veterinary Research and Quarantine Service
2College of Veterinary Medicine, Konkuk National University
국내분리 오제스키병 바이러스의 게놈 유전자 특성 분석
현방훈1, 김인중1, 표현미1, 차상호1, 박지연1, 송재영1, 조인수1, 양창범1, 안수환1, 이중복2
1국립수의과학검역원 동물위생연구소
2건국대학교 수의과대학
Abstract
The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.
Key Words: Aujeszky's disease virus, characterization, genome, Korean isolate


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