| Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis |
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Ji Hye Lee1, Keun Jung Kim1, Seon A Choi1, Xiaoxia Li1, Eun Young Kim1, Hyun Ju Oh2, Byeong Chun Lee2, Hye Jin Kim3, Byung Kwon Park4, Min Kyu Kim1 |
1College of Agriculture and Life Sciences, Chungnam National University
2College of Veterinary Medicine, Seoul National University
3Haemaru Small Animal Referral Hospital
4College of Industrial Science, Kongju National University |
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| Abstract |
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This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{ imes}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch. |
| Key Words:
frozen-thawed semen, intrauterine insemination, microsatellite, superfecundation |
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