Korean Journal of Veterinary Research 2009;49(4):319-328.
Random amplification of polymorphic DNA typing of Listeria monocytogenes isolates from animal products
Chul-Hyun Lee1, Won-Geun Son2
1National Veterinary Research and Quarantine Service
2College of Veterinary Medicine and Veterinary Medical Research Institute
축산물유래 Listeria monocytogenes의 RAPD typing
이철현1, 손원근2
1국립수의과학검역원
2제주대학교 수의과대학 및 수의과학연구소
Abstract
This study investigated the epidemiology of Listeria (L.) monocytogenes, a food-borne pathogen. The epidemiology of food-borne pathogens is of great importance for clarifying bacterial origin and preventing bacterial contamination and infection. This work examined 68 L. monocytogenes strains, including 11 reference strains and 57 isolates from imported US beef, domestic meats (beef, pork, chicken meat), raw milk, and milk plants. The random amplified polymorphic DNA (RAPD) techniques were optimized to develop a standard molecular epidemiological analysis of L. monocytogenes. There was great genetic variability among the isolates, which produced 24 and 34 RAPD patterns with primer HLWL85 and HLWL74, respectively. The discriminatory power of the RAPD methods with HLWL85 and HLWL74 primer were very high (DI = 0.957; S ${geq}$ 80%, S ${geq}$ 95%). Some RAPD types were specific to origin. A few RAPD types were specific for L. monocytogenes strains belonging to a particular serotype. Using the HLWL85 primer, the strains isolated from milk plants could be distinguished from the other strains. And using the HLWL74 primer, the strains isolated from imported beef (US) could be distinguished completely from the other strains.
Key Words: Listeria monocytogenes, molecular epidemiology, RAPD typing
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