Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea

Kim, Jeong-Hwa; Lim, Jeong Ju; Kim, Dong Hyeok; Lee, Jin Ju; Kim, Dae Geun; Jun, Moo-Hyung; Kim, Sang Hun; Chang, Hong Hee; Lee, Hu Jang; Min, Won-Gi; Kim, Suk

Korean Journal of Veterinary Research 2010;50(2):117-124.

Jeong-Hwa Kim¹, Jeong Ju Lim², Dong Hyeok Kim², Jin Ju Lee², Dae Geun Kim², Moo-Hyung Jun³, Sang Hun Kim³, Hong Hee Chang⁴, Hu Jang Lee², Won-Gi Min², Suk Kim²

¹Northern Branch, Gyeongbuk Veterinary Service Laboratory
²College of Veterinary Medicine, Gyeongsang National University
³College of Veterinary Medicine, Chungnam National University
⁴Institute of Agriculture and Life Science, Gyeongsang National University

국내 경북지역 소에서 분리된 브루셀라 분리주의 생물학적 특성

김정화¹, 임정주², 김동혁², 이진주², 김대근², 전무형³, 김상훈³, 장홍희⁴, 이후장², 민원기², 김석²

¹경상북도 가축위생시험소 북부지소
²경상대학교 수의과대학
³충남대학교 수의과대학
⁴경상대학교 농업생명과학연구원

Abstract

Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

Key Words: Brucella abortus, Brucellosis, cattle, isolation, PCR