| Evaluation of character on lymphocyte subpopulations from peripheral blood in Hanwoo and Holstein neonatal calves |
|
Young-Hun Jung1, Tai-Young Hur1, Seog-Jin Kang1, Kwang-Seok Ki1, Sung-Jae Park1, Myeung-Sik Lee1, Guk-hyun Suh2 |
1National Institute of Animal Science, Rural Development Administration
2College of Veterinary Medicine, Chonnam National University |
| 한우와 젖소 신생 송아지의 말초혈액 림프구아군 특성비교 |
|
정영훈1, 허태영1, 강석진1, 기광석1, 박성재1, 이명식1, 서국현2 |
1농촌진흥청 국립축산과학원
2전남대학교 수의과대학 |
|
|
| Abstract |
|
The present study was undertaken to establish reference values for the composition blood lymphocyte populations and compare forty three Hanwoo neonatal calves (KC) with twenty one Holstein calves (HC) by blood cell count and immunophynotying. The percentages of CD2+, CD4+, CD8+, CD26+, ACT2+, MHC class, MHC class II and WC1+ T cells, B cells were determined by flow cytometry. The number of lymphocyte and monocyte in HC were higher than those of KC. However, the number of neutrophils was higher in HC than KC. The proportions of CD2+, CD4+, CD8+, MHC class, and WC1+ lymphocytes remained relatively stable during the study period, while there was a moderate increase in the relative percentage of CD26+, ACT2+, MHC class II and B cell from birth to approximately 3 weeks of age. Marked differences in the relative proportions of the lymphocyte subpopulations were noted between the individual calves. The present study shows that the T-cell subpopulations are present in peripheral blood of KC at levels comparable with HC, while the MHC class II and B cell population of KC increases significantly with age. The absolute number of WBC in KC was due to the decrease of absolute number of neutrophil rather than the increase of lymphocyte. The results indicated that KC have significantly higher number of neutrophils, and proportion of MHC class II and B cell than HC. |
| Key Words:
flow cytometry, Hanwoo and Holstein calf, immunophynotype, lymphocyte subpopulation |
|
PDF Links
Full text via DOI
Download Citation
