Korean Journal of Veterinary Research 2010;50(4):285-295.
Proteome analysis between diverse phenotypes of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium)
Gee-Wook Shin1, In-Seok Cha2, Woo-Won Lee3, Seong-Won Nho2, Seong-Bin Park2, Ho-Bin Jang2, Yong-Hwan Kim2, Tae-Sung Jung2
1College of Veterinary Medicine, Chonbuk National University
2College of Veterinary Medicine, Gyeongsang National University
3Busan Metropolitan City Institute of Health and Environmental
Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.
Key Words: OMP, phage type, plasmid, proteome, Salmonella enterica serovar Typhimurium, Triton X-114

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