Korean Journal of Veterinary Research 2010;50(4):331-333.
Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium
Casiano H. Jr. Choresca1, Dennis K. Gomez2, Jee-Eun Han1, Sang-Phil Shin1, Ji-Hyung Kim1, Jin-Woo Jun1, Se-Chang Park1
1Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University
2Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines in the Visayas
Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
Key Words: aerolysin gene, Aeromonas hydrophila, catfish, extracellular hemolysin gene

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