| Quantification and genotyping of PCV2 DNA in the tissues of PCV2-infected conventional pigs with different clinical signs |
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Hye Kwon Kim1, Yuzi Luo1, Hyoung Joon Moon2, Seong Jun Park1, Se Mi Rho1, Jae Yeon Han1, Van Giap Nguyen1, Bong Kyun Park1 |
1College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University
2Research Unit, Green Cross Veterinary Products |
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| Abstract |
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This study was focused on the genotyping and quantification of Porcine circovirus type 2 (PCV2) in thirty PCV2-positive pigs with different clinical symptoms (PCV2-infected without wasting, PCV2-infected with wasting, PCV2-infected with wasting and lymphoid depletion). The quantity of PCV2 DNA in diverse tissues was significantly differed among these groups. (One-way ANOVA test, p<0.001) Interestingly, PCV2-DNA load in tissues of PCV2-infected pigs without wasting and PCV2-infected pigs with wasting and lymphoid depletion were not significantly differed (p = 0.38), while they were all significantly higher when compared with PCV2-infected pigs with wasting-only. PCV2 DNA quantity in tissues was significantly higher in PCV2a and 2b co-infected pigs compared to the PCV2b only-infected pigs (Wilcoxon test, p = 0.039). The PCV2a and 2b co-infected pigs had increased wasting and lymphoid depletion rate but it was not statistically significant. Therefore, this cross-sectional study suggested that PCV2 DNA load in tissues was diverse by clinical and histological findings. Furthermore, co-infection of PCV2a and 2b affected to the PCV2 DNA load in tissues with increased rate of wasting and lymphoid depletion. |
| Key Words:
clinical sign, genotype, PCV2a, PCV2b, quantification |
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