The virulence of Brucella abortus isolated from cattle in Korea |
Jeong Ju Lim1, Jeong-Hwa Kim2, Dong Hyeok Kim1, Jin Ju Lee1, Dae Geun Kim1, Moo-Hyung Jun3, Sang Hun Kim3, Hong Hee Chang4, Hu Jang Lee1, Won-Gi Min1, Suk Kim1 |
1College of Veterinary Medicine, Gyeongsang National University 2Northern Branch, Gyeongbuk Veterinary Service Laboratory 3College of Veterinary Medicine, Chungnam National University 4College of Institute of Agriculture and Life Science, Gyeongsang National University |
국내 소에서 분리한 Brucella abortus의 병원성 분석 |
임정주1, 김정화2, 김동혁1, 이진주1, 김대근1, 전무형3, 김상훈3, 장홍희4, 이후장1, 민원기1, 김석1 |
1경상대학교 수의과대학 2경상북도 가축위생시험소 북부지소 3충남대학교 수의과대학 4경상대학교 농업생명과학연구원 |
|
Abstract |
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class I, lower internalization than that of B. abortus 544; class II, similar internalization to that of that of B. abortus 544; class III, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class III showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression. |
Key Words:
Brucella abortus, cattle, phagocytes, virulence |
|