Korean J Vet Res > Volume 51(2); 2011 > Article
Korean Journal of Veterinary Research 2011;51(2):129-137.
DOI: https://doi.org/10.14405/kjvr.2011.51.2.129    Published online June 1, 2011.
Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR
Hye Kwon Kim1, Hyoung Joon Moon2, Seong Jun Park1, Se Mi Rho1, Jae Yeon Han1, Van Giap Nguyen1, Bong Kyun Park1
1College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University
2Research Unit, Green Cross Veterinary Products
Abstract
Swine diseases could be caused by unrecognized or minor pathogens. In this study, two unknown cytopathogenic agents were isolated from swine, through cell culture. In order to identify these two cytopathogenic agent (designated CP129 and #2045-7), a particle associated nucleic acids PCR (PANPCR) from previous paper was used with simple modification. The cloning procedure was more specified in this study by adding cell control system. According to the modified PAN-PCR, two and four agentsspecific DNA sequences were obtained from CP129 and #2045-7, respectively, and they were identified as Mycoplasma (M.) hyorhinis and Mammalian orthoreovirus by nucleotide BLAST. Since M. hyorhinis (CP129) was filterable and non-visible by microscope, this unusual virus-like nature of M. hyorhinis (CP129) was discussed. Especially, the reovirus (#2045-7) was a serotype 3 and a triple reassortant among three serotypes of reoviruses. It was grouped with recently reported reoviruses from disease cases (swine, human and feline), based on the genetic analysis of L1 and S1 partial sequences. In conclusion, two unknown cytopathogenic agents were successfully identified using modified PAN-PCR with cell control system and they were characterized in this study.
Key Words: mammalian orthoreovirus, Mycoplasma hyorhinis, PAN-PCR, pig, unknown
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