| Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus |
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Kang-Seuk Choi1, Woo-Jin Jeon1, Eun-Kyoung Lee2, Soo-Jeong Kye1, Mi-Ja Park1, Jun-Hun Kwon1 |
1Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency
2Veterinary Medicine and Biologics Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency |
| 한국호흡기형 닭전염성기관지염 생독백신주의 작성 |
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최강석1, 전우진1, 이은경2, 계수정1, 박미자1, 권준헌1 |
1농림수산검역검사본부 동식물위생연구부 조류질병과
2농림수산검역검사본부 동식물위생연구부 동물약품평가과 |
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| Abstract |
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An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea. |
| Key Words:
attenuation, infectious bronchitis, live vaccine, protection |
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